Super-enhancer Acquisition Drives FOXC2 Expression in Middle …
WEBApr 16, 2021 · We then investigated H3K27ac ChIP-seq to map the active cis-regulatory landscape in human cholesteatoma. Based on the results, increased H3K27ac at the FOXC2 gene locus—one among the Fox families—was observed in the cholesteatoma specimens and FOXC2 protein expression was confirmed in the human cholesteatoma tissues immunohistochemically ...
Structural basis for DNA recognition by FOXC2 - PMC
WEBApr 4, 2019 · Given the high identity between FOXC2 and FOXC1, we analyzed a deposited FOXC1 ChIP-seq dataset (ERP006190). For motif RYAAACA, 7721 peaks were found (Figure (Figure7A), 7A ), accounting for 32.3% of total FOXC1 binding sites.
Super-enhancer Acquisition Drives FOXC2 Expression in Middle …
WEBApr 16, 2021 · Among the detected genes in ChIP-seq, which were shown in Fig. 2e, an increased H3K27ac signal of the SE associated with FOXC2 was observed in the cholesteatoma specimens compared with the results for the skin specimens (Fig. 2G).
FOXC2 controls formation and maturation of lymphatic collecting vessels ...
WEBWe used chromatin immunoprecipitation (ChIP) coupled with tiling microarrays covering the entire human genome (ChIP-chip) to produce an unbiased high resolution map of FOXC2-binding sites. For each ChIP-chip biological replicate, the immunoprecipitation was verified by Western blotting with anti-FOXC2 antibody ( Fig. S4 A ).
Super-Enhancer-Associated Transcription Factors Maintain ...
WEBJun 1, 2021 · Because FOXC1/2 specifically expressed in podocytes in glomeruli, we performed ChIP-seq of FOXC1 and FOXC2 in adult mice glomeruli (Supplemental Tables 9 and 10). Metagene analysis showed that bindings of both FOXC were enriched at the transcription start site ( Supplemental Figure 10A ).
Fast and flexible profiling of chromatin accessibility and total RNA ...
WEBMar 26, 2024 · Single cell chromatin accessibility profiling and transcriptome sequencing are the most widely used technologies for single-cell genomics. Here, we present Microwell-seq3, a high-throughput and...
Structural basis for DNA recognition by FOXC2 - ResearchGate
WEBApr 23, 2019 · The FOXC2-DBD adopts the winged-helix fold with helix H3 contributing to all the base specific contacts, while the N-terminus, wing 1, and the C-terminus of FOXC2-DBD all make additional...
WEBFOXC2) plays essential roles in the regulation of em-bryonic, ocular, and cardiac development. Mutations and abnormal expression of FOXC proteins are im-plicated in genetic diseases as well as cancer. In this study, we determined two crystal structures of the DNA-binding domain (DBD) of human FOXC2 pro-tein,incomplexwithdifferentDNAsites.TheFOXC2-
Genetic variations of DNA bindings of FOXA1 and co-factors in ... - Nature
WEBSep 13, 2021 · Here, the authors develop a statistical framework to analyse ChIP-seq and GWAS data, identify 22 breast cancer risk-associated TFs and a core TF-transcriptional network for FOXA1 and...
FOXC2 promotes vasculogenic mimicry and resistance to anti …
WEBAug 29, 2023 · Here we show that the transcription factor, Foxc2, promotes VM in diverse solid tumor types by driving ectopic expression of endothelial genes in tumor cells, a process that is stimulated by hypoxia. VM-proficient tumors are resistant to anti-angiogenic therapy, and suppression of Foxc2 augments response.