(G) The representative RT-PCR results of isoform II and isoform I in our OSCC or normal samples. GAPDH served as a loading control. (H-I) The scatter dot plot summarized the ratio of isoform II versus ...

PCR reactions were normalised by expression analysis of GAPDH or cytokeratin 18 (CK18) with the following primers: GAPDH forward, 5′-ATCAGCAATGCCTCCTGCAC-3′, and reverse, 5′-TGGCATGGACTGTGGTCATG-3′; ...

Furthermore, real-time reverse transcription polymerase chain reaction (RT-PCR) tests for both housekeeping genes (GAPDH and β -actin) and RPE-specific genes (MITF, PEDF, and PMEL17) show no ...

Subsequently, real-time polymerase chain reaction (PCR) was used to analyze ... role in cell growth and brain development, ...

Quantitative PCR were performed using One Step TB Green PrimeScript RT ... 3’ for ORC1 and 5’- TCCACTGGCGTCTTCACC-3’, 5’-GGCAGAGATGATGACCCTTTT-3’ for GAPDH. GAPDH was used as a control for calculating ...

Using the 2 −∆∆Cq approach, the miRNA expression levels were measured and normalized to the internal reference genes, namely U6 and GAPDH. The cyclic conditions used in the q-PCR system were as ...

All PCR reactions were supplemented with 10 μL of DNA dilution ... FFPE samples often exhibit numerous non-amplifications attributable to paraffin defects and DNA damage. However, GAPDH consistently ...

PCR-Machines are based on Polymerase Chain Reaction (PCR) and produce copies of genes exponentially. The PCR cycling reactions are carried out in an automated thermocycler, which is capable of ...

IMDX Monkeypox Detection RT-PCR Assay by Siemens Healthineers has received manufacturing approval from the Central Drugs Standard Control Organisation (CDSCO) The World Health Organisation (WHO ...

Activation of macrophage TLR4 by bacterial endotoxins is important for the progression of sepsis, but the underlying ...